Enantioseparation of Chiral Phenoxy Acid Derivatives Using Polysaccharide Based Chiral Stationary Phase under Polar Organic Mobile Phase Conditions

Author: ana ghibradze
Co-authors: Bezhan Chankvetadze
Keywords: The enantiomeric analysis of chiral derivatives of phenoxypropionic acid by HPLC using polysacharide
Annotation:

Chiral phenoxy acid derivatives represent an important group of biologically active compounds. Until now no systematic study has been reported on the enantiomer elution order of this group of chemical compounds in chiral HPLC, as well as their separation with polar organic mobile phases has not reported at all. In the present study, the separation of enantiomers of 18 representatives of this group of chiral compounds was studied on polysaccharide-based chiral columns. As the mobile phase alcohols (methanol and ethanol) and acetonitrile with 0.1% (v/v) additive of formic acid were used. Five different polysaccharide-based chiral columns of Lux series, such as Lux Cellulose-1, Lux Celluose-2, lux Cellulose-3, Lux Cellulose-4 and Lux Amylose-2 from Phenomenex (Torrance, CA, USA) and SP-6 Amylose-tris(3,5-dimethylphenylcarbamate) were used (Fig.1). The separations were performed using Agilent 1260 HPLC instrument equipped with a binary pump and variable wavelength UV-detector. In alcohols as mobile phases the best results were obtained on SP-6 Amylose-tris(3,5-dimethylphenylcarbamate), Lux Cellulose-3 and Cellulose-1 and columns. In acetonitrile as a mobile phase the Lux Cellulose-1 separated the highest number of studied compounds. Few examples of enantiomer elution order reversal depending on the chiral selector and the mobile phase were observed.