Study of stability of G4 Dendrimer and DNA complexes by calorimetric method

Author: Mariami Shubitidze
Keywords: DNA, Dendrimer
Annotation:

Were studied PAMAM G4 double circuit and its DNA interaction with the thermodynamic properties of molecules of Dendrimer. As a result of study, has been revealed that DNA molecules form a complex with Dendrimer. About complex formation indicates experiments obtained by micro-calorimetric method. Namely, it is clearly visible that when mixing Dendimer with DNA molecule, the Dendimer predominantly is connected with rich areas of pairs of Guanine – Cytosis in DNA molecules. About this fact indicates the DNA melting curve in the temperature interval, significant reduction in small size peak characterized for Guanine – Cytosis, so that the melting curve does not suffer other changes. In addition was studied DNA - Dendrimer complex features of a variety acidity of (pH 4.6-8.0) environmental conditions. The research shows that pH has a significant influence on DNA - Dendrimer complex. Namely, takes place enthalpy meaning change on pH direction by melting point

Lecture files:

G4 დენდრიმერისა და დნმ-ის კომპლექსის სტაბილობის კალორიმეტრული კვლევები [ka]